Identification of novel dysregulated key genes in Breast cancer through high throughput ChIP-Seq data analysis

Sci Rep. 2017 Jun 12;7(1):3229. doi: 10.1038/s41598-017-03534-x.


Breast cancer is the most common cancer in women both in the developed and less developed countries, and it imposes a considerable threat to human health. Therefore, in order to develop effective targeted therapies against Breast cancer, a deep understanding of its underlying molecular mechanisms is required. The application of deep transcriptional sequencing has been found to be reported to provide an efficient genomic assay to delve into the insights of the diseases and may prove to be useful in the study of Breast cancer. In this study, ChIP-Seq data for normal samples and Breast cancer were compared, and differential peaks identified, based upon fold enrichment (with P-values obtained via t-tests). The Protein-protein interaction (PPI) network analysis was carried out, following which the highly connected genes were screened and studied, and the most promising ones were selected. Biological pathway involved in the process were then identified. Our findings regarding potential Breast cancer-related genes enhances the understanding of the disease and provides prognostic information in addition to standard tumor prognostic factors for future research.

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Line
  • Cell Line, Tumor
  • Chromatin Immunoprecipitation
  • Computational Biology / methods*
  • Female
  • Genetic Predisposition to Disease / genetics
  • Genomics / methods*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • MCF-7 Cells
  • Protein Interaction Maps / genetics*
  • Risk Factors


  • Biomarkers, Tumor