Zymography Principles

Methods Mol Biol. 2017;1626:3-10. doi: 10.1007/978-1-4939-7111-4_1.


Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are routinely detected by zymography, especially with clinical interest. This introductory chapter reviews the major principles behind zymography. New advances of this method are basically focused towards two-dimensional zymography and transfer zymography as will be explained in the rest of the chapters. Some general considerations when performing the experiments are outlined as well as the major troubleshooting and safety issues necessary for correct development of the electrophoresis.

Keywords: Electrophoresis; Enzymes; Troubleshooting; Zymography.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocatalysis
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Enzyme Assays / methods*
  • Fluorescent Dyes / analysis
  • Humans
  • Indicators and Reagents
  • Oxidation-Reduction
  • Proteomics / methods


  • Fluorescent Dyes
  • Indicators and Reagents