Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies

Cell. 1985 Jul;41(3):697-706. doi: 10.1016/s0092-8674(85)80050-7.


Exposure of neu-oncogene-transformed NIH 3T3 cells to monoclonal antibodies reactive with the neu gene product, p185, results in the rapid and reversible loss of both cell-surface and total cellular p185. Although not directly cytotoxic, monoclonal anti-p185 antibody treatment causes neu-transformed NIH 3T3 cells to revert to a nontransformed phenotype, as determined by anchorage-independent growth. Isotype matched control antibodies of an unrelated specificity do not affect p185 levels or colony formation in soft agar by neu-transformed NIH 3T3 cells. Soft agar colony formation by NIH 3T3 cells transformed by ras oncogenes is not affected by anti-p185 antibody treatment. Anchorage-independent growth of cells from the ethylnitrosourea-induced rat neuroblastoma line in which neu was originally detected by DNA transfection is also inhibited in the presence of anti-p185 monoclonal antibodies. Collectively, these results suggest that p185 is required to maintain transformation induced by the neu oncogene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Division
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Cell Transformation, Viral
  • Clone Cells
  • Harvey murine sarcoma virus / physiology
  • Mice
  • Neuroblastoma / pathology
  • Oncogenes*
  • Phenotype
  • Phosphoproteins / immunology
  • Phosphoproteins / physiology*
  • Receptor, ErbB-2
  • Transfection


  • Antibodies, Monoclonal
  • Phosphoproteins
  • Receptor, ErbB-2