Objective: To explore the influence of smoking and polycyclic aromatic hydrocarbons (PAHs) on urinary 8-hydroxydeoxyguanosine (8-OHdG) in coke oven workers and investigate their dose-dependent relationships. Methods: A total of 436 workers exposed to coke oven emissions (COEs) and 132 controls were recruited in this study. Questionnaires were completed in a personal interview. Then their urine samples were also collected and the concentrations of urinary four OH-PAHs and 8-OHdG were determined by high performance liquid chromatography (HPLC) which was used to evaluate the levels of occupational PAHs internal exposure among workers and the DNA damage. Results: The differences of concentrations of urinary 2-NAP (2-hydroxynathalene) , 2-FLU (2-hydroxyfluorene) , 9-PHE (9-hydroxyphenanthrene) , 1-OHP (1-hydroxypyrene) between exposure group and control group were statistically significant (P<0.05) . In exposure group and control group, the level of 8-OHdG in heavy smoking workers were significantly higher than that in other groups (P<0.05) . Multivariate logistic regression analysis revealed high levels of urinary 8-OHdG were associated with a significantly increased risk of having higher urinary1-hydroxypyrene levels[OR=1.43 (95%CI: 1.06-1.94) , P<0.01] and heavy smoking [OR=1.44 (95%CI: 1.08-1.91) , P<0.01], respectively. Trend test showed that linear dose response relationship between smoking, 1-OHP in urine and higher concentrations of 8-OHdG (P<0.05) . Smoking could significant modify the effects of urinary 1-hydroxypyrene, while co-exposure to both heavy smoking and high urinary 1-hydroxypyrene levels[OR=5.64 (95%CI: 2.15-14.80) , P<0.05]. Conclusion: Urinary 1-hydroxypyrene is a useful biomarker for evaluating total PAHs exposure, coke oven workers with heavy smoking present more serious DNA oxditive damage.
目的： 探讨吸烟与多环芳烃（polycyclic aromatic hydrocarboins，PAHs）暴露对焦炉作业工人尿中8-羟基脱氧鸟苷（8-hydroxydeoxyguanosine，8-OHdG）的影响及剂量-反应关系。 方法： 选取某焦化厂焦炉工436人作为接触组，非焦炉工132人作为对照组，利用问卷调查一般情况，以高效液相色谱法测定晨尿中4种PAHs代谢物水平和氧化损伤标志物8-OHdG水平。 结果： 接触组尿中2-羟基萘（2-hydroxynathalene，2-NAP）、2-羟基芴（2-hydroxyfluorene，2-FLU）、9-羟基菲（9-hydroxyphenanthrene，9-PHE）和1-羟基芘（1-hydroxypyrene，1-OHP）与对照组比较，均增高（P<0.05）；在接触组和对照组中均发现重度吸烟工人尿中8-OHdG含量高于轻度吸烟和不吸烟的工人（P<0.05）；多因素logistic回归分析显示，吸烟和尿中1-羟基芘与尿中高浓度8-OHdG的发生密切相关，OR值分别1.44（95%CI：1.08~1.91）和1.43（95%CI：1.06~1.94）（P<0.01）；趋势检验显示，吸烟程度和尿中1-羟基芘浓度与尿中高浓度8-OHdG的发生具有线性剂量-反应关系（P<0.05）；多因素logistic回归分析显示，吸烟与1-羟基芘对尿中8-OHdG含量有交互作用，1-羟基芘高暴露重度吸烟组工人发生高浓度8-OHdG的可能性是1-羟基芘低暴露不吸烟组的5.64倍（95% CI：2.15~14.80）（P<0.05）。 结论： 吸烟和PAHs暴露对焦炉作业工人尿中8-OHdG含量有交互作用。在焦炉作业工人中采取控烟措施对预防氧化损伤引起的肿瘤等相关疾病的发生具有重要意义。.
Keywords: 1-hydroxypyrene; 8-hydroxydeoxyguanosine; Chromatography, high pressure liquid; Coke oven workers; Polycyclic aromatic hydrocarbons; Smoking.