New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR

Arch Biochem Biophys. 2017 Aug 15;628:102-113. doi: 10.1016/j.abb.2017.06.010. Epub 2017 Jun 13.

Abstract

Magic angle spinning solid state NMR studies of biological macromolecules [1-3] have enabled exciting studies of membrane proteins [4,5], amyloid fibrils [6], viruses, and large macromolecular assemblies [7]. Dynamic nuclear polarization (DNP) provides a means to enhance detection sensitivity for NMR, particularly for solid state NMR, with many recent biological applications and considerable contemporary efforts towards elaboration and optimization of the DNP experiment. This review explores precedents and innovations in biological DNP experiments, especially highlighting novel chemical biology approaches to introduce the radicals that serve as a source of polarization in DNP experiments.

Keywords: Dynamic nuclear polarization; In cell NMR; Nitroxide biradical; Paramagnetic tags; Solid state NMR.

Publication types

  • Review
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Proteins / chemistry*
  • Proteins / metabolism*

Substances

  • Proteins