Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

Bioresour Technol. 2017 Dec;245(Pt B):1400-1406. doi: 10.1016/j.biortech.2017.05.190. Epub 2017 Jun 1.


This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, β-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources.

Keywords: Cell factory; Glutathione; Mannan-based bioresource; Mannanase/mannosidase-expressing yeast; Synthetic biology.

MeSH terms

  • Glutathione
  • Mannans*
  • Saccharomyces cerevisiae
  • beta-Mannosidase


  • Mannans
  • beta-1,4-mannan
  • beta-Mannosidase
  • Glutathione