Different patterns of expression of cell cycle control and local invasion-related proteins in oral squamous cell carcinoma affecting young patients

J Oral Pathol Med. 2018 Jan;47(1):32-39. doi: 10.1111/jop.12601. Epub 2017 Aug 4.

Abstract

Objectives: Oral squamous cell carcinoma (OSCC) predominantly affects males in the fifth decade of life; nevertheless, an increased incidence in young patients has been reported worldwide, and the clinical and behavioral characteristics of tumors in this group are controversial, and the literature shows divergent results.

Purpose: To investigate the clinicopathological features and prognostic significance of the immunoexpression of cell cycle and local invasion proteins in OSCC affecting young patients (≤40 years old).

Methods: A tissue microarray was performed with 132 OSCC samples (61 cases of young patients vs 71 cases of elderly patients) and submitted to immunohistochemical reactions with Ki67, p53, p16, Bcl-2, Cyclin D1, C-ErbB2, p21, Myc, EGFR, MMP-9, SMA, Cathepsin K and FGF-2 antibodies.

Results: Clinicopathological features and survival rates were similar in both groups. Although overexpression of EGFR (P=.042) and MMP-9 (P=.001) was more frequent in young patients, only C-ErbB-2 (P=.048) and SMA (P=.048) expression correlated with lower disease-free survival (DFS) in this group of patients.

Conclusion: Clinicopathological features and survival rates are similar between younger and older patients with OSCC. The different patterns of C-ErbB2, EGFR, MMP-9, and SMA expression between the groups merits further investigation to understand their role in the early tumor onset in young patients.

Keywords: cell cycle proteins; clinicopathologic characteristics; local invasion proteins; oral squamous cell carcinoma.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Brazil
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cathepsin K / metabolism
  • Cell Cycle Checkpoints / genetics
  • Cell Cycle Checkpoints / physiology*
  • Cyclin D1 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Disease-Free Survival
  • Epithelial Cells / pathology
  • Erb-b2 Receptor Tyrosine Kinases / metabolism
  • ErbB Receptors / metabolism
  • Female
  • Fibroblast Growth Factor 2 / metabolism
  • Gene Expression Regulation, Neoplastic / genetics
  • Gene Expression Regulation, Neoplastic / physiology*
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / metabolism
  • Male
  • Matrix Metalloproteinase 9 / metabolism
  • Middle Aged
  • Mouth Neoplasms / genetics
  • Mouth Neoplasms / metabolism*
  • Mouth Neoplasms / mortality
  • Mouth Neoplasms / pathology
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Prognosis
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Proto-Oncogene Proteins c-myc / metabolism
  • Survival Rate
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Cathepsin K
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor p16
  • Cyclin-Dependent Kinase Inhibitor p21
  • ErbB Receptors
  • Fibroblast Growth Factor 2
  • Ki-67 Antigen
  • Matrix Metalloproteinase 9
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins c-myc
  • Erb-b2 Receptor Tyrosine Kinases
  • Tumor Suppressor Protein p53
  • BCL2 protein, human
  • CCND1 protein, human
  • CDKN1A protein, human
  • CDKN2A protein, human
  • MYC protein, human
  • EGFR protein, human
  • ERBB2 protein, human
  • CTSK protein, human