Biomimetic electrospun scaffolds from main extracellular matrix components for skin tissue engineering application - The role of chondroitin sulfate and sulfated hyaluronan

Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:15-22. doi: 10.1016/j.msec.2017.05.005. Epub 2017 May 4.

Abstract

Incorporation of bioactive components like glycosaminoglycans (GAGs) into tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion. The effects of these scaffolds were analyzed on human keratinocyte (HaCaT), fibroblast (Hs27) and mesenchymal stem cells (hMSCs) adhesion and proliferation. A significant increase in cell number (~5 fold) was observed when cultivating all three cell types alone on scaffolds containing sHA and CS. These findings suggest that sulfated GAG-containing electrospun nanofibrous scaffolds might be beneficial for the development of effective skin tissue engineered constructs by stimulating cellular performance and therefore accelerate epidermal-dermal regeneration processes.

Keywords: Cellular adhesion and proliferation; Electrospun nanofibrous scaffolds; Sulfated glycosaminoglycans.

MeSH terms

  • Biomimetics
  • Cell Proliferation
  • Cells, Cultured
  • Chondroitin Sulfates
  • Extracellular Matrix
  • Humans
  • Hyaluronic Acid
  • Skin
  • Tissue Engineering*
  • Tissue Scaffolds

Substances

  • Hyaluronic Acid
  • Chondroitin Sulfates