Detection of an Adherence Factor of Enteropathogenic Escherichia Coli With a DNA Probe

J Infect Dis. 1985 Sep;152(3):560-5. doi: 10.1093/infdis/152.3.560.

Abstract

A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coli isolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF] was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adhesins, Escherichia coli
  • Adhesiveness
  • Bacterial Proteins / genetics*
  • Cell Line
  • DNA, Bacterial
  • Diarrhea / microbiology
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Escherichia coli / pathogenicity
  • Escherichia coli / physiology
  • Humans
  • Infant
  • Nucleic Acid Hybridization
  • Plasmids*
  • Serotyping

Substances

  • Adhesins, Escherichia coli
  • Bacterial Proteins
  • DNA, Bacterial