Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

Mol Ther. 2017 Aug 2;25(8):1736-1738. doi: 10.1016/j.ymthe.2017.05.021. Epub 2017 Jun 17.

Abstract

CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Chromosome Deletion*
  • Embryonic Stem Cells / metabolism
  • Gene Editing
  • Gene Targeting*
  • Mice
  • RNA, Guide
  • Zygote

Substances

  • RNA, Guide