Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells

J Immunol. 2017 Aug 1;199(3):874-884. doi: 10.4049/jimmunol.1700431. Epub 2017 Jun 21.


TCR stimulation by peptide-MHC complexes on APCs requires precise reorganization of molecules into the area of cellular contact to form an immunological synapse from where T cell signaling is initiated. Caveolin (Cav)1, a widely expressed transmembrane protein, is involved in the regulation of membrane composition, cellular polarity and trafficking, and the organization of signal transduction pathways. The presence of Cav1 protein in T cells was identified only recently, and its function in this context is not well understood. We show that Cav1-knockout CD8 T cells have a reduction in membrane cholesterol and sphingomyelin, and upon TCR triggering they exhibit altered morphology and polarity, with reduced effector function compared with Cav1 wild-type CD8 T cells. In particular, redistribution of the β2 integrin LFA-1 to the immunological synapse is compromised in Cav1-knockout T cells, as is the ability of LFA-1 to form high-avidity interactions with ICAM-1. Our results identify a role for Cav1 in membrane organization and β2 integrin function in primary CD8 T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / chemistry
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Caveolin 1 / deficiency
  • Caveolin 1 / metabolism*
  • Cell Membrane / chemistry
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cell Polarity / immunology
  • Cholesterol / analysis
  • Immunological Synapses / chemistry
  • Immunological Synapses / immunology
  • Immunological Synapses / metabolism*
  • Intercellular Adhesion Molecule-1 / metabolism
  • Lymphocyte Activation*
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Mice
  • Receptors, Antigen, T-Cell / chemistry
  • Receptors, Antigen, T-Cell / immunology*
  • Signal Transduction
  • Sphingomyelins / analysis


  • Caveolin 1
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Antigen, T-Cell
  • Sphingomyelins
  • Intercellular Adhesion Molecule-1
  • Cholesterol