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, 14 (4), 169-179
eCollection

EFFICIENCY OF BORAGE SEEDS OIL AGAINST GAMMA IRRADIATION-INDUCED HEPATOTOXICITY IN MALE RATS: POSSIBLE ANTIOXIDANT ACTIVITY

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EFFICIENCY OF BORAGE SEEDS OIL AGAINST GAMMA IRRADIATION-INDUCED HEPATOTOXICITY IN MALE RATS: POSSIBLE ANTIOXIDANT ACTIVITY

Hala A H Khattab et al. Afr J Tradit Complement Altern Med.

Abstract

Background: Borage (Borago officinal L.) is an annual herbaceous plant of great interest because its oil contains a high percentage of γ-linolenic acid (GLA). The present work was carried out to detect fatty acids composition of the oil extracted from borage seeds (BO) and its potential effectiveness against γ-irradiation- induced hepatotoxicity in male rats.

Materials and methods: GC-MS analysis of fatty acids methyl esters of BO was performed to identify fatty acids composition. Sixty rats were divided into five groups (12 rats each): Control, irradiated; rats were exposed to (6.5 Gy) of whole body γ-radiation, BO (50 mg/kg b.wt), irradiated BO post-treated and irradiated BO prepost-treated. Six rats from each group were sacrificed at two time intervals 7 and 15 days post-irradiation. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT) levels, lipids profile, as well as serum and hepatic reduced glutathione (GSH) and lipid peroxide (malondialdehyde) (MDA) levels were assessed. Histopathological examination of liver sections were also carried out.

Results: The results showed that the high contents of BO extracted by cold pressing, were linoleic acid (34.23%) and GLA (24.79%). Also, oral administration of BO significantly improved serum levels of liver enzymes, lipids profile, as well as serum and hepatic GSH and MDA levels (p<0.001) as compared with irradiated rats after 15 days post irradiation. Moreover, it exerted marked amelioration against irradiation-induced histopathological changes in liver tissues. The improvement was more pronounced in irradiated BO prepost-treated group than irradiated BO post-treated.

Conclusion: BO has a beneficial role in reducing hepatotoxicity and oxidative stress induced by radiation exposure. Therefore, BO may be used as a beneficial supplement for patients during radiotherapy treatment.

Keywords: Antioxidant; Borage seeds oil; Hepatotoxicity; γ-irradiation.

Figures

Figure 1
Figure 1
GC-MS of fatty acids methyl esters of oil extracted from borage seeds
Figure 2
Figure 2
Liver sections from control showing normal structure (1). Liver sections of irradiated rats at the 7th day showing hepatic necrosis area associated and replaced with leucocytic inflammatory cells (2-a &b). while at the 15th day showing focal hydropic degeneration of hepatocytes and portal infiltration with mononuclear inflammatory cells (3-a) (H & E x 200), with focal perivascular leucocytic cells infiltration (arrow) (3-b) (H & E x 400).
Figure 3
Figure 3
Liver from BO group showing no histopathological alterations (1). Liver sections of irradiated BO post-treated rats at the 7th day showing hepatoportal blood vessels congestion (small arrows), as well as portal infiltration with mononuclear cells (large arrow) (2), while at the 15th day showing apparent normal structure (3). Sections of irradiated BO prepost- treated rats at the 7th day showing slight dilatation of hepatic sinusoids (small arrow) with few leucocytes in hepatic sinusoids and binucleation of hepayocytes (large arrows) (4) (H&E x 400), while at the 15th day showing normal histological structure (5) (H & E x 200).

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References

    1. Adams RB. Identification of essential oil compounds by gas chromatography mass spectroscopy. Allured Pub. Corp, Carol. Stream; Illinois USA: 1995.
    1. Arafa H.M, Abd-Ellah M.F, Hafez H.F. Abatement by naringenin of doxorubic-induced cardiac toxicity in rats. J. Egypt. Nat. cancer Inst. 2005;17(4):291–300. - PubMed
    1. Asadi-Samani M, Bahmani M, Rafieian-Kopaei M. The chemical composition, botanical characteristic and biological activities of Borago officinalis: a review. Asian Pac J Trop Med. 2014;7S1:S22–28. doi:10.1016/S1995-7645(14)60199-1. - PubMed
    1. Ashry OM, Hussein EM. Radioprotective potency of ginseng on some haematopoeitic and physiological parameters in irradiated rats. Egypt J. of Rad. Sci. Applic. 2007;20(1):39–53.
    1. Baker JE, Fish BL, Su J, Haworth ST, Strande JL, Komorowski RA, Migrino RQ, Doppalapudi A, Harmann L, Allen LX, Hopewell JW, Moulder JE. 10 Gy total body irradiation increases risk of coronary sclerosis, degeneration of heart structure and function in a rat model. Int. J. Radiat. Biol. 2009;85(12):1089–1100. - PMC - PubMed

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