Detection of MicroRNA in Hepatic Cirrhosis and Hepatocellular Carcinoma in Hepatitis C Genotype-4 in Egyptian Patients

Biomed Res Int. 2017:2017:1806069. doi: 10.1155/2017/1806069. Epub 2017 May 23.

Abstract

Background: In Egypt, the prevalence of chronic hepatitis C (CHC) infection is 13.8% of whole population and about 80% of the patients with hepatocellular carcinoma have underling hepatitis C.

Aim: This study was designed to assess the diagnostic value of plasma miR-122 and miR-21 in patients with CHC, genotype-4, to detect fibrosis progression versus noninvasive indices and their diagnostic value in detection of early stages of hepatocellular carcinoma (HCC).

Methodology: A prospective study that included 180 patients, divided into 3 groups: healthy controls (group I), CHC patients (group II), and hepatitis C patients with HCC (group III); all cases were subjected to thorough clinical, radiological, and laboratory investigations. Selected biomarkers were evaluated and correlated with degree of liver damage. Results revealed that miR-122 followed by miR-21 had the highest efficiency in prediction of liver cell damage. Also, miR-21 was strongly correlated with vascular endothelial growth factor (VEGF) and alpha fetoprotein (α-FP) in HCC patients.

Conclusions: Plasma miR-122 and miR-21 had strong correlation with degree fibrosis in HCV genotype-4 patients; consequently they can be considered as potential biomarker for early detection of hepatic fibrosis. Moreover, miR-21 can be used as a potential biomarker, for early detection of HCC combined with VEGF and α-FP.

MeSH terms

  • Adult
  • Biomarkers, Tumor / blood
  • Carcinoma, Hepatocellular / blood*
  • Carcinoma, Hepatocellular / epidemiology
  • Carcinoma, Hepatocellular / pathology
  • Egypt / epidemiology
  • Female
  • Genotype
  • Hepacivirus / pathogenicity
  • Hepatitis C, Chronic / blood*
  • Hepatitis C, Chronic / epidemiology
  • Hepatitis C, Chronic / pathology
  • Humans
  • Liver Cirrhosis / blood*
  • Liver Cirrhosis / epidemiology
  • Liver Cirrhosis / pathology
  • Liver Neoplasms / blood*
  • Liver Neoplasms / epidemiology
  • Liver Neoplasms / pathology
  • MicroRNAs / blood*
  • Middle Aged
  • Vascular Endothelial Growth Factor A / blood
  • alpha-Fetoproteins / metabolism

Substances

  • Biomarkers, Tumor
  • MIRN122 microRNA, human
  • MIRN21 microRNA, human
  • MicroRNAs
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • alpha-Fetoproteins