Generation of Knock-in Mouse by Genome Editing

Methods Mol Biol. 2017;1630:91-100. doi: 10.1007/978-1-4939-7128-2_8.

Abstract

Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using embryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-stranded oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.

Keywords: Engineered endonuclease; Genome editing; Knock-in mice; Single-stranded oligodeoxynucleotide (ssODN); Zygote.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Single-Stranded / genetics
  • Endonucleases / metabolism
  • Gene Editing / methods*
  • Gene Knock-In Techniques / methods*
  • Mice
  • Microinjections
  • Oligodeoxyribonucleotides / genetics
  • Zygote / growth & development

Substances

  • DNA, Single-Stranded
  • Oligodeoxyribonucleotides
  • Endonucleases