Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease

Yasunori Sasakura; Keita Yoshida; Nicholas Treen

doi:10.1007/978-1-4939-7128-2_19

Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease

Methods Mol Biol. 2017;1630:235-245. doi: 10.1007/978-1-4939-7128-2_19.

Authors

Yasunori Sasakura¹, Keita Yoshida², Nicholas Treen²

Affiliations

¹ Shimoda Marine Research Center, University of Tsukuba, 5-10-1, Shimoda, Shizuoka, 415-0025, Japan. sasakura@shimoda.tsukuba.ac.jp.
² Shimoda Marine Research Center, University of Tsukuba, 5-10-1, Shimoda, Shizuoka, 415-0025, Japan.

PMID: 28643263
DOI: 10.1007/978-1-4939-7128-2_19

Abstract

The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.

Keywords: Ascidian; Ciona intestinalis; Electroporation; Genome editing; Knockout; TALEN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ciona intestinalis / genetics*
Electroporation
Gene Editing / methods*
Gene Knockout Techniques
Organ Specificity
Transcription Activator-Like Effector Nucleases / metabolism

Substances

Transcription Activator-Like Effector Nucleases