A comparative study of the type-3 fimbriae of Klebsiella species

J Med Microbiol. 1985 Oct;20(2):203-14. doi: 10.1099/00222615-20-2-203.

Abstract

Type-3 fimbriae isolated from members of five different species of Klebsiella were 4-5 nm in diameter and agglutinated the tannic acid-treated erythrocytes of ox and, in some cases, the untanned erythrocytes of fowl. In sodium dodecyl sulphate-polyacrylamide gel electrophoresis, the type-3 fimbrial proteins had mol. wts in the range 19 500-21 500. Hydrophobic amino acids comprised 39.6% of all the amino acids of the type-3 fimbrial protein of K. oxytoca strain 70/1. The type-1 fimbrial protein of Klebsiella had a mol. wt of c. 18 000 and the type-1 fimbriae were serologically distinct from the type-3 fimbriae. Our results for the type-3 fimbriae of Klebsiella were compared with those of others for morphologically similar and serologically related thin fimbriae of Salmonella and Yersinia.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acids / analysis
  • Antigens, Bacterial / immunology
  • Bacterial Proteins / analysis*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Fimbriae, Bacterial / analysis*
  • Fimbriae, Bacterial / immunology
  • Fimbriae, Bacterial / ultrastructure
  • Hemagglutination Tests
  • Hemagglutinins / immunology
  • Klebsiella / ultrastructure*
  • Klebsiella pneumoniae / ultrastructure*
  • Microscopy, Electron
  • Molecular Weight
  • Species Specificity

Substances

  • Amino Acids
  • Antigens, Bacterial
  • Bacterial Proteins
  • Hemagglutinins