Mass Spectrometry Imaging, Laser Capture Microdissection, and LC-MS/MS of the Same Tissue Section

J Proteome Res. 2017 Aug 4;16(8):2993-3001. doi: 10.1021/acs.jproteome.7b00284. Epub 2017 Jul 5.


Mass spectrometry imaging (MSI) is able to simultaneously record the distributions of hundreds of molecules directly from tissue. Rapid direct tissue analysis is essential for MSI in order to maintain spatial localization and acceptable measurement times. The absence of an explicit analyte separation/purification step means MSI lacks the depth of coverage of LC-MS/MS. In this work, we demonstrate how atmospheric pressure MALDI-MSI enables the same tissue section to be first analyzed by MSI, to identify regions of interest that exhibit distinct molecular signatures, followed by localized proteomics analysis using laser capture microdissection isolation and LC-MS/MS.

Keywords: laser capture microdissection; localized microproteomics; mass spectrometry imaging; microproteomics; molecular histology; multimodal analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Atmospheric Pressure
  • Chromatography, Liquid
  • Humans
  • Imaging, Three-Dimensional / methods*
  • Laser Capture Microdissection / methods*
  • Proteomics / methods*
  • Spatial Analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Tandem Mass Spectrometry
  • Time Factors
  • Tissue Distribution