Alternative RNA Splicing Associated With Mammalian Neuronal Differentiation

Cereb Cortex. 2018 Aug 1;28(8):2810-2816. doi: 10.1093/cercor/bhx160.


Alternative pre-mRNA splicing (AS) produces multiple isoforms of mRNAs and proteins from a single gene. It is most prevalent in the mammalian brain and is thought to contribute to the formation and/or maintenance of functional complexity of the brain. Increasing evidence has documented the significant changes of AS between different regions or different developmental stages of the brain, however, the dynamics of AS and the possible function of it during neural progenitor cell (NPC) differentiation is less well known. Here, using purified NPCs and their progeny neurons isolated from the embryonic mouse cerebral cortex, we characterized the global differences of AS events between the 2 cell types by deep sequencing. The sequencing results revealed cell type-specific AS in NPCs and neurons that are important for distinct functions pertinent to the corresponding cell type. Our data may serve as a resource useful for further understanding how AS contributes to molecular regulations in NPCs and neurons during cortical development.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alternative Splicing
  • Animals
  • Cell Differentiation / genetics*
  • Cells, Cultured
  • Cerebral Cortex / cytology*
  • Computational Biology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Doublecortin Domain Proteins
  • Embryo, Mammalian
  • Gene Expression Regulation, Developmental / genetics
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Mice
  • Mice, Transgenic
  • Microarray Analysis
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Nestin / genetics
  • Nestin / metabolism
  • Neural Stem Cells / physiology*
  • Neurons / physiology*
  • Neuropeptides / genetics
  • Neuropeptides / metabolism
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • RNA, Messenger / metabolism
  • Ubiquitin-Protein Ligases


  • DNA-Binding Proteins
  • Doublecortin Domain Proteins
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • Nestin
  • Neuropeptides
  • Nuclear Proteins
  • RNA, Messenger
  • Green Fluorescent Proteins
  • Trim27 protein, mouse
  • Ubiquitin-Protein Ligases