Feeding of the water extract from Ganoderma lingzhi to rats modulates secondary bile acids, intestinal microflora, mucins, and propionate important to colon cancer

Biosci Biotechnol Biochem. 2017 Sep;81(9):1796-1804. doi: 10.1080/09168451.2017.1343117. Epub 2017 Jun 29.


Consumption of reishi mushroom has been reported to prevent colon carcinogenesis in rodents, although the underlying mechanisms remain unclear. To investigate this effect, rats were fed a high-fat diet supplemented with 5% water extract from either the reishi mushroom (Ganoderma lingzhi) (WGL) or the auto-digested reishi G. lingzhi (AWGL) for three weeks. Both extracts markedly reduced fecal secondary bile acids, such as lithocholic acid and deoxycholic acid (colon carcinogens). These extracts reduced the numbers of Clostridium coccoides and Clostridium leptum (secondary bile acids-producing bacteria) in a per g of cecal digesta. Fecal mucins and cecal propionate were significantly elevated by both extracts, and fecal IgA was significantly elevated by WGL, but not by AWGL. These results suggest that the reishi extracts have an impact on colon luminal health by modulating secondary bile acids, microflora, mucins, and propionate that related to colon cancer.

Keywords: Ganoderma lingzhi; colon; mucins; secondary bile acids; short-chain fatty acids.

MeSH terms

  • Animals
  • Bile Acids and Salts / metabolism*
  • Body Weight / drug effects
  • Cecum / drug effects
  • Cecum / microbiology
  • Colonic Neoplasms* / metabolism
  • Colonic Neoplasms* / microbiology
  • Diet
  • Eating / drug effects
  • Fatty Acids, Volatile / metabolism
  • Feces / chemistry
  • Ganoderma / chemistry*
  • Gastrointestinal Microbiome / drug effects*
  • Immunoglobulin A / metabolism
  • Male
  • Mucins / metabolism*
  • Propionates / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Water / chemistry*


  • Bile Acids and Salts
  • Fatty Acids, Volatile
  • Immunoglobulin A
  • Mucins
  • Propionates
  • Water