Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed

PLoS One. 2017 Jun 29;12(6):e0180053. doi: 10.1371/journal.pone.0180053. eCollection 2017.


A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were non-motile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).

MeSH terms

  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Nucleic Acid Hybridization
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seaweed / microbiology*
  • Vibrio / classification
  • Vibrio / genetics*


  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Grants and funding

This work was supported by Kaken (16H0497606) (TS). This work was also supported by Grant in Aid for Scientific Research on Innovative Area “Genome Science” from Ministry of Education, Culture, Sports, Science, and Technology of Japan (No.221S0002).