Use of streptavidin to detect biotin-containing proteins in plants

Anal Biochem. 1985 Sep;149(2):448-53. doi: 10.1016/0003-2697(85)90596-2.


A procedure to detect biotinyl proteins after fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was developed. Proteins were immobilized on nitrocellulose and biotin-containing proteins were detected by probing with 125I-streptavidin. Using this procedure a small survey of biotinyl protein in plants was undertaken. In total four biotin-containing proteins were detected in higher plants of molecular weights 62,000, 50,000, 34,000, and 31,000. These biotinyl proteins were not ubiquitous in the plants surveyed. In the cyanobacterium Anabeana variabilis, a single biotin-containing protein of 21,000 Da was detected. In isolated spinach chloroplasts, the two biotinyl proteins detected were soluble. The results are discussed in relation to acetyl-CoA carboxylase.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetyl-CoA Carboxylase / metabolism
  • Bacterial Proteins*
  • Biotin / isolation & purification*
  • Chloroplasts / analysis
  • Collodion
  • Electrophoresis, Polyacrylamide Gel
  • Plant Extracts / analysis
  • Plant Proteins / isolation & purification*
  • Streptavidin


  • Bacterial Proteins
  • Plant Extracts
  • Plant Proteins
  • Biotin
  • Collodion
  • Streptavidin
  • Acetyl-CoA Carboxylase