Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection

ZhiHong Li; GuoQing Pan; ZhenGang Ma; Bing Han; Bin Sun; Qi Ni; Jie Chen; Tian Li; TongBao Liu; MengXian Long; ChunFeng Li; ZeYang Zhou

doi:10.1016/j.jip.2017.06.009

Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection

J Invertebr Pathol. 2017 Oct:149:36-43. doi: 10.1016/j.jip.2017.06.009. Epub 2017 Jun 28.

Authors

ZhiHong Li¹, GuoQing Pan¹, ZhenGang Ma², Bing Han¹, Bin Sun¹, Qi Ni¹, Jie Chen¹, Tian Li¹, TongBao Liu¹, MengXian Long¹, ChunFeng Li³, ZeYang Zhou⁴

Affiliations

¹ The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China.
² College of Life Sciences, Chongqing Normal University, Chongqing 400047, China.
³ The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China. Electronic address: licf@swu.edu.cn.
⁴ The State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China; College of Life Sciences, Chongqing Normal University, Chongqing 400047, China.

PMID: 28668257
DOI: 10.1016/j.jip.2017.06.009

Abstract

Nosema bombycis is an obligate intracellular parasite, which can cause pébrine disease. To investigate the effects of N. bombycis infection, 5th-instar silkworms were challenged with N. bombycis isolate CQ1, and two-dimensional gel electrophoresis analysis was performed to analyze the differentially expressed proteins in infected and uninfected silkworm fat bodies 1, 2, 4, 6 and 8days post-infection (dpi). 46 differentially expressed proteins were identified at the 5 time points using MALDI-TOF/TOF MS. The changed proteins mainly involved in immune response, energy metabolism, and molecular synthesis. Overall, the identified proteins may provide important insights into the mechanisms of the silkworm response to N. bombycis infection.

Keywords: Host responses; Nosema bombycis; Proteome; Silkworm.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bombyx / metabolism*
Bombyx / microbiology*
Fat Body / metabolism*
Microsporidiosis / metabolism*
Nosema / physiology*
Proteomics