Lipopolysaccharides Derived from Pantoea agglomerans Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells

Anticancer Res. 2017 Jul;37(7):3917-3920. doi: 10.21873/anticanres.11774.


Background/aim: Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line.

Materials and methods: Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte™ Fluor 488-conjugated Aβ1-42).

Results: The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ1-42 phagocytosis, LPSp significantly increased Aβ phagocytic activity.

Conclusion: LPSp treatment was confirmed to enhance Aβ1-42 phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer's disease.

Keywords: Lipopolysaccharide; Pantoea agglomerans; amyloid β; microglia; toll-like receptor.

MeSH terms

  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Cell Line
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Humans
  • Lipopolysaccharides / pharmacology*
  • Mice
  • Microglia / drug effects*
  • Microglia / metabolism
  • Pantoea / metabolism*
  • Peptide Fragments / metabolism*
  • Phagocytosis


  • Amyloid beta-Peptides
  • Lipopolysaccharides
  • Peptide Fragments
  • amyloid beta-protein (1-42)
  • lipopolysaccharide IP-PA1, Pantoea agglomerans