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. 2017 Jul;7(3):217.
doi: 10.1007/s13205-017-0835-1. Epub 2017 Jul 1.

Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30

Affiliations

Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30

Ushakiranmayi Managamuri et al. 3 Biotech. 2017 Jul.

Abstract

Deep sea sediment samples of Bay of Bengal (Visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. The actinomycetes strain VSM-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. It was identified as Streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. Response surface methodology regression analysis was carried out to fit the experimental data of each response by the second-order polynomial. The results have proven right interaction among process variables at optimized values of incubation time at 12 days, pH at 8, temperature at 30 °C, concentrations of starch at 1%, and tryptone at 1% and the data have been adequately fitted into the second-order polynomial models. Under these conditions, the responses (zones of inhibition) of plant pathogenic fungi Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Fusarium solani, and Penicillium citrinum were also matched with experimental and predicted results. Chemotypic analysis of ethyl acetate extract of the strain was done using LC-Q-TOF-MS revealed the presence of bioactive compounds including tryptophan dehydrobutyrine diketopiperazine, maculosin, 7-o-demethyl albocycline, albocycline M-2, and 7-o-demethoxy-7-oxo albocycline in a negative ion mode. The ethyl acetate extract of actinobacterium has been subjected to gas chromatography and mass spectroscopy (GC-MS) revealed the presence of diverse compounds such as dotriacontane, tetracosane 11-decyl-, diheptyl phthalate, 1-hexadecanesulfonyl chloride, L-alanyl-L-tryptophan, phthalic acid ethyl pentyl ester, 4-trifluoroacetoxyhexadecane, and 1H-imidazole 4,5-dihydro-2,4-dimethyl. Hence, the ethyl acetate extract of Streptomyces sparsus VSM-30 may have antibacterial, antifungal, and antioxidant activities due to the presence of secondary metabolites in ethyl acetate extract. The study also supports marine sediment samples of Bay of Bengal, a promising marine ecosystem remained to be explored for new bioactive compounds.

Keywords: Actinobacterium; Bioactive metabolites; Regression analysis; Response surface methodology; Streptomyces sparsus.

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Conflict of interest statement

All authors declare that there is no conflict of interest.

Figures

Fig. 1
Fig. 1
Scanning electron micrograph of the marine actinobacterium VSM-30 grown on ISP-2 medium
Fig. 2
Fig. 2
Evolutionary relationships of taxa
Fig. 3
Fig. 3
3D plots showing the interactive effects of selective variables on the production of the bioactive metabolites by Streptomyces sparsus VSM-30 and its effect against the response Aspergillus niger (zone of inhibition in mm)
Fig. 4
Fig. 4
3D plots showing the interactive effects of selective variables on the production of the bioactive metabolites by Streptomyces sparsus VSM-30 and its effect against the response Aspergillus flavus (zone of inhibition in mm)
Fig. 5
Fig. 5
3D plots showing the interactive effects of selective variables on the production of the bioactive metabolites by Streptomyces sparsus VSM-30 and its effect against the response, Fusarium oxysporum (zone of inhibition in mm)
Fig. 6
Fig. 6
3D plots showing the interactive effects of selective variables on the production of the bioactive metabolites by Streptomyces sparsus VSM-30 and its effect against the response Fusarium solani (zone of inhibition in mm)
Fig. 7
Fig. 7
3D plots showing the interactive effects of selective variables on the production of the bioactive metabolites by Streptomyces sparsus VSM-30 and its effect against the response Penicillium citrinum (zone of inhibition in mm)
Fig. 8
Fig. 8
Negative ion (−) ESI–MS/MS analysis of ethyl acetate extract of strain VSM-30
Fig. 9
Fig. 9
Negative ion (−) ESI–MS/MS of ethyl acetate extract of strain VSM-30
Fig. 10
Fig. 10
Gas-chromatography–mass spectrometry analysis of crude ethyl acetate extract of VSM-30

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