Enzymatic saccharification of lignocellulosic residues using cellulolytic enzyme extract produced by Penicillium roqueforti ATCC 10110 cultivated on residue of yellow mombin fruit

Abstract

The aim of this work was to enzymatic saccharification of food waste was performed by crude enzymatic cellulolytic extract produced by P. roqueforti cultivated in yellow mombin residue. The best yield of reducing sugars (259.45mgg^-1) was achieved with sugarcane bagasse after 4h; the hydrolysis of corn cob, rice husk and peanut hull resulted in yields around 128-180mgg^-1. The addition of 10mmolL^-1 of Mn²⁺ potentiated the saccharification of sugarcane bagasse, in about 86%. The temperature and substrate (sugarcane bagasse) concentration parameters were optimized using a Doehlert Design and, a maximum sugar yield of 662.34±26.72mgg^-1 was achieved at 62.40°C, 0.22% (w/v) of substrate, with the addition of Mn²⁺. Sugar yield was significantly high when compared to previous studies available in scientific literature, suggesting the use of crude cellulolytic supplemented with Mn²⁺ an alternative and promising process for saccharification of sugarcane bagasse.

Keywords: Corn cob; Enzymatic hydrolysis; Metal ion; Optimization; Peanut hull; Rice husk; Sugarcane bagasse.