The effect of tomatine on metastasis related matrix metalloproteinase (MMP) activities in breast cancer cell model

Gene. 2017 Sep 5;627:408-411. doi: 10.1016/j.gene.2017.06.054. Epub 2017 Jun 29.


Breast cancer is one of the most common malignancies in women and metastasis is the cause of morbidity and mortality in patients. In the development of metastasis, the matrix metalloproteinase (MMP) family has a very important role in tumor development. MMP-2 and MMP-9 work together for extracellular matrix (ECM) cleavage to increase migration. Tomatine is a secondary metabolite that has a natural defense role against plants, fungi, viruses and bacteria that are synthesized from tomato. In additıon, tomatine is also known that it breaks down the cell membrane and is a strong inhibitor in human cancer cells. In this study, it was aimed to evaluate the effect of tomatine on cytotoxicity, apoptosis and matrix metalloproteinase inhibition in MCF-7 cell lines. Human breast cancer cell line (MCF-7) was used as a cell line. In MCF-7 cells, the IC50 dose of tomatine was determined to be 7.07μM. According to the control cells, apoptosis increased 3.4 fold in 48thh. Activation of MMP-2, MMP-9 and MMP-9\NGAL has been shown to decrease significantly in cells treated with tomatine by gelatin zymography compared to the control. As a result, matrix metalloproteinase activity and cell proliferation were suppressed by tomatine and this may provide support in treatment methods.

Keywords: Breast cancer; Matrix metalloproteinase; Tomatine.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Breast Neoplasms / metabolism*
  • Cell Proliferation / drug effects
  • Humans
  • MCF-7 Cells
  • Matrix Metalloproteinases / genetics*
  • Matrix Metalloproteinases / metabolism
  • Tomatine / pharmacology*


  • Antineoplastic Agents
  • Tomatine
  • Matrix Metalloproteinases