First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

Juan Wang; Min Yuan; Hai Chen; Xia Chen; Yuanchun Jia; Xiong Zhu; Li Bai; Xue Bai; Séamus Fanning; Jinxing Lu; Juan Li

doi:10.1128/AAC.00877-17

First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

Antimicrob Agents Chemother. 2017 Aug 24;61(9):e00877-17. doi: 10.1128/AAC.00877-17. Print 2017 Sep.

Authors

Juan Wang^{1

2}, Min Yuan³, Hai Chen⁴, Xia Chen³, Yuanchun Jia³, Xiong Zhu⁴, Li Bai⁵, Xue Bai¹, Séamus Fanning^{5

2

6}, Jinxing Lu³, Juan Li⁷

Affiliations

¹ College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
² UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield, Dublin, Ireland.
³ Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
⁴ People's Hospital of Sanya, Hainan Province, China.
⁵ Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing, China.
⁶ Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.
⁷ Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China lijuan@icdc.cn.

Abstract

The nucleotide sequences of five plasmids from one Klebsiella oxytoca isolate were determined using the PacBio RS II system. Plasmid analysis revealed that bla_NDM-1 was carried on an IncX3 plasmid. The bla_IMP-4 and bla_KPC-2 genes were located on IncN and IncP-6 plasmids, respectively. Comparative sequence analysis highlighted the successful spread of carbapenemase-harboring plasmids among different enterobacterial species. We report for the first time, to our knowledge, coproducing NDM-1, KPC-2, and IMP-4 carbapenemases on a K. oxytoca isolate.

Keywords: KPC-2; MBLs; MDR Klebsiella oxytoca; carbapenemases; plasmids.

MeSH terms

Aged
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Enterobacteriaceae / drug effects
Enterobacteriaceae / genetics
Enterobacteriaceae Infections / drug therapy
Humans
Klebsiella Infections / drug therapy
Klebsiella oxytoca / drug effects
Klebsiella oxytoca / genetics*
Microbial Sensitivity Tests / methods
Plasmids / genetics
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-lactamase IMP-4
beta-lactamase KPC-2
beta-Lactamases
beta-lactamase NDM-1
carbapenemase