Primary structure and expression of a functional human glucocorticoid receptor cDNA

Nature. 1985 Dec 19;318(6047):635-41. doi: 10.1038/318635a0.


Identification of complementary DNAs encoding the human glucocorticoid receptor predicts two protein forms, of 777 (alpha) and 742 (beta) amino acids, which differ at their carboxy termini. The proteins contain a cysteine/lysine/arginine-rich region which may define the DNA-binding domain. Pure radiolabelled glucocorticoid receptor, synthesized in vitro, is immunoreactive and possesses intrinsic steroid-binding activity characteristic of the native glucocorticoid receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation*
  • Humans
  • Macromolecular Substances
  • Poly A / analysis
  • RNA, Messenger / analysis
  • Receptors, Glucocorticoid / genetics*


  • Macromolecular Substances
  • RNA, Messenger
  • Receptors, Glucocorticoid
  • Poly A
  • DNA

Associated data

  • GENBANK/M10901
  • GENBANK/M11050