Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis Without Depletion of Abundant Proteins

Methods Mol Biol. 2017;1619:119-125. doi: 10.1007/978-1-4939-7057-5_9.

Abstract

Immunoaffinity enrichment combined with LC-MS/MS enables identification of Post-translational modifications (PTMs) from serum/plasma samples without abundant protein depletion. Here we described the workflow in details in identifying various types of PTMs such as lysine acetylation and arginine methylation from cancer serum. The method described is compatible with all common proteomic analysis platforms and quantitative methods.

Keywords: Immunoaffinity purification (IAP); LC-MS/MS; Post-translational modification (PTM); Serum/plasma.

MeSH terms

  • Blood Proteins* / chemistry
  • Blood Proteins* / isolation & purification
  • Chromatography, Liquid*
  • Humans
  • Protein Processing, Post-Translational*
  • Proteome*
  • Proteomics* / methods
  • Tandem Mass Spectrometry*

Substances

  • Blood Proteins
  • Proteome