Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry

Methods Mol Biol. 2017:1619:203-212. doi: 10.1007/978-1-4939-7057-5_16.


Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 μL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.

Keywords: Blood plasma; LipidXplorer; Lipids; Mass spectrometry; Shotgun lipidomics.

MeSH terms

  • Humans
  • Lipids / blood*
  • Lipids / isolation & purification
  • Mass Spectrometry* / methods
  • Mass Spectrometry* / standards
  • Metabolome*
  • Metabolomics* / methods
  • Metabolomics* / standards
  • Reference Standards
  • Reproducibility of Results
  • Software
  • Statistics as Topic
  • Workflow


  • Lipids