Single cell transcriptomics of pluripotent stem cells: reprogramming and differentiation

Curr Opin Genet Dev. 2017 Oct;46:66-76. doi: 10.1016/j.gde.2017.06.003. Epub 2017 Jul 1.

Abstract

Single-cell transcriptomics serves as a powerful tool to identify cell states within populations of cells, and to dissect underlying heterogeneity at high resolution. Single-cell transcriptomics on pluripotent stem cells has provided new insights into cellular variation, subpopulation structures and the interplay of cell cycle with pluripotency. The single-cell perspective has helped to better understand gene regulation and regulatory networks during exit from pluripotency, cell-fate determination as well as molecular mechanisms driving cellular reprogramming of somatic cells to induced pluripotent stage. Here we review the recent progress and significant findings from application of single-cell technologies on pluripotent stem cells along with a brief outlook on new combinatorial single-cell approaches that further unravel pluripotent stem cell states.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Differentiation / genetics*
  • Cellular Reprogramming / genetics*
  • Gene Expression Regulation, Developmental
  • Humans
  • Pluripotent Stem Cells / metabolism*
  • Single-Cell Analysis / methods*