An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Stem Cell Res. 2017 May;21:5-8. doi: 10.1016/j.scr.2017.03.011. Epub 2017 Mar 15.

Abstract

The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism*
  • Gene Transfer Techniques*
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism*
  • Macaca mulatta
  • Male
  • Mice
  • Mice, SCID
  • Transcription Factors* / biosynthesis
  • Transcription Factors* / genetics

Substances

  • Transcription Factors