Multisite Electrophysiology Recordings in Mice to Study Cross-Regional Communication During Anxiety

Curr Protoc Neurosci. 2017 Jul 5:80:8.40.1-8.40.21. doi: 10.1002/cpns.32.


Recording neural activity in awake, freely moving mice is a powerful and flexible technique for dissecting the neural circuit mechanisms underlying pathological behavior. This unit describes protocols for designing a drive and recording single neurons and local field potentials during anxiety-related paradigms. We also include protocols for integrating pharmacologic and optogenetic means for circuit manipulations, which, when combined with electrophysiological recordings, demonstrate input-specific and cell-specific contributions to circuit-wide activity. We discuss the planning, execution, and troubleshooting of physiology experiments during anxiety-like behavior. © 2017 by John Wiley & Sons, Inc.

Keywords: anxiety; implant construction; implant design; in vivo electrophysiology; local field potentials; multisite recordings.

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Anxiety / pathology
  • Anxiety / physiopathology*
  • Cell Communication / physiology*
  • Electrodes, Implanted
  • Electrophysiology / instrumentation
  • Electrophysiology / methods*
  • Mice
  • Nerve Net / physiology*
  • Neurons / physiology*
  • Optogenetics
  • Wakefulness