Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

PLoS One. 2017 Jul 5;12(7):e0180347. doi: 10.1371/journal.pone.0180347. eCollection 2017.

Abstract

We have characterized nine mcr-1-harboring plasmids from clinical Escherichia coli isolates previously described in Argentina and Canada. Three of these plasmids carried a mcr-1-variant called here mcr-1.5. All these E. coli isolates were not clonally related and were recovered in different years and locations. However, their mcr-1-harboring plasmids showed high identity among them and to others characterized in other countries, which strongly suggests that this plasmid-type is playing an important role in spreading this mechanism of resistance to polymyxins.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Argentina
  • Canada
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / transmission
  • Escherichia coli Proteins / genetics*
  • Ethanolaminephosphotransferase / genetics*
  • Genetic Variation
  • Genotype
  • Humans
  • Microbial Sensitivity Tests
  • Plasmids / chemistry
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • Polymyxins / pharmacology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Escherichia coli Proteins
  • MCR-1 protein, E coli
  • Polymyxins
  • Ethanolaminephosphotransferase

Grant support

The work was supported by internal budget, Public Health Ontario.