Assessment of the Therapeutic Potential of Persimmon Leaf Extract on Prediabetic Subjects

Mol Cells. 2017 Jul 31;40(7):466-475. doi: 10.14348/molcells.2017.2298. Epub 2017 Jul 6.


Dietary supplements have exhibited myriads of positive health effects on human health conditions and with the advent of new technological advances, including in the fields of proteomics, genomics, and metabolomics, biological and pharmacological activities of dietary supplements are being evaluated for their ameliorative effects in human ailments. Recent interests in understanding and discovering the molecular targets of phytochemical-gene-protein-metabolite dynamics resulted in discovery of a few protein signature candidates that could potentially be used to assess the effects of dietary supplements on human health. Persimmon (Diospyros kaki) is a folk medicine, commonly used as dietary supplement in China, Japan, and South Korea, owing to its different beneficial health effects including anti-diabetic implications. However, neither mechanism of action nor molecular biomarkers have been discovered that could either validate or be used to evaluate effects of persimmon on human health. In present study, Mass Spectrometry (MS)-based proteomic studies were accomplished to discover proteomic molecular signatures that could be used to understand therapeutic potentials of persimmon leaf extract (PLE) in diabetes amelioration. Saliva, serum, and urine samples were analyzed and we propose that salivary proteins can be used for evaluating treatment effectiveness and in improving patient compliance. The present discovery proteomics study demonstrates that salivary proteomic profile changes were found as a result of PLE treatment in prediabetic subjects that could specifically be used as potential protein signature candidates.

Keywords: biomarker candidates; diabetes; mass spectrometry; persimmon leaf extract; proteomics; saliva; serum; urine.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Biomarkers / metabolism
  • Blotting, Western
  • Cytoskeletal Proteins / metabolism
  • Demography
  • Diospyros / chemistry*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Phytotherapy
  • Placebos
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Plant Leaves / chemistry*
  • Prediabetic State / drug therapy*
  • Prediabetic State / metabolism
  • Principal Component Analysis
  • Proteome / metabolism
  • Saliva / metabolism
  • Tandem Mass Spectrometry


  • Biomarkers
  • Cytoskeletal Proteins
  • Placebos
  • Plant Extracts
  • Proteome
  • ezrin