Enzyme activity evaluation by differential electrochemical mass spectrometry

João C P de Souza; Wanderson O Silva; Fabio H B Lima; Frank N Crespilho

doi:10.1039/c7cc03963h

Enzyme activity evaluation by differential electrochemical mass spectrometry

Chem Commun (Camb). 2017 Jul 25;53(60):8400-8402. doi: 10.1039/c7cc03963h.

Authors

João C P de Souza¹, Wanderson O Silva², Fabio H B Lima², Frank N Crespilho²

Affiliations

¹ São Carlos Institute of Chemistry, University of São Paulo, São Carlos, SP 13560-970, Brazil. frankcrespilho@iqsc.usp.br and Goiano Federal Institute, Rio Verde, GO 75901-970, Brazil.
² São Carlos Institute of Chemistry, University of São Paulo, São Carlos, SP 13560-970, Brazil. frankcrespilho@iqsc.usp.br.

PMID: 28681865
DOI: 10.1039/c7cc03963h

Abstract

NAD-dependent alcohol dehydrogenase (ADH) enzymes for ethanol oxidation were investigated by differential electrochemical mass spectrometry (DEMS). The broad mass spectra obtained under bioelectrochemical control and with unprecedented accuracy were used to provide new insight into the enzyme kinetics and mechanisms.