Characterization of the O-antigen polysaccharide derived from Pantoea agglomerans IG1 lipopolysaccharide

Masahito Hashimoto; Rune Satou; Mami Ozono; Hiroyuki Inagawa; Gen-Ichiro Soma

doi:10.1016/j.carres.2017.06.017

Characterization of the O-antigen polysaccharide derived from Pantoea agglomerans IG1 lipopolysaccharide

Carbohydr Res. 2017 Sep 8:449:32-36. doi: 10.1016/j.carres.2017.06.017. Epub 2017 Jun 28.

Authors

Masahito Hashimoto¹, Rune Satou², Mami Ozono², Hiroyuki Inagawa³, Gen-Ichiro Soma³

Affiliations

¹ Department of Chemistry, Biotechnology, and Chemical Engineering, Kagoshima University, Kagoshima, Japan. Electronic address: hassy@eng.kagoshima-u.ac.jp.
² Department of Chemistry, Biotechnology, and Chemical Engineering, Kagoshima University, Kagoshima, Japan.
³ Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Kagawa, Japan; Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Akiba-ku, Niigata, Japan; Control of Innate Immunity TRA, Bio-Lab, Kagawa, Japan.

PMID: 28686930
DOI: 10.1016/j.carres.2017.06.017

Abstract

A polysaccharide fraction was isolated from the Pantoea agglomerans IG1 lipopolysaccharide (IP-PA1), and its O-antigenic polysaccharide was characterized by chemical analyses and 1D and 2D ¹H and ¹³C NMR spectroscopy. The polysaccharide is composed of linear tetrasaccharide repeating units, consisting of glucose and rhamnose, where 40% of one of the rhamnose residues is substituted with glucose: →2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]_0.4-α-l-Rhap-(1→2)-α-l-Rhap-(1→.

Keywords: Lipopolysaccharide; NMR spectroscopy; O-antigen polysaccharide; Pantoea agglomerans; Wheat symbiotic bacteria.

MeSH terms

Carbohydrate Sequence
O Antigens / chemistry*
Pantoea / chemistry*

Substances

O Antigens