Amino acid composition in determination of collagen origin and assessment of physical factors effects

Int J Biol Macromol. 2017 Nov;104(Pt A):987-991. doi: 10.1016/j.ijbiomac.2017.07.013. Epub 2017 Jul 4.

Abstract

The amino acid composition of collagen is a characteristic feature of this protein. Collagen, irrespective of its origin, contains 19 amino acids, including hydroxyproline which does not occur in other proteins. Its atypical amino acid composition is characterized by high content of proline and glycine, as well as the absence of cysteine. This paper shows the comparison of qualitative composition of amino acids of fish skin (FS) collagen, bovine Achilles tendon (BAT) collagen, and bone collagen. Results demonstrate that FS collagen as well as BAT collagen contains no cysteine and significantly different amount of hydroxyproline. In BAT collagen hydroxyproline content is 30% higher than hydroxyproline content of FS collagen. In bone collagen the amount of hydroxyproline is two times more than in FS collagen. Furthermore, it is shown that sensitivity to radiation of individual amino acids varies and depends on the absorbed dose of ionizing radiation. The changes observed in the amino acid composition become very intense for the doses of 500kGy and 1000kGy.

Keywords: Bone collagen; Fish skin collagen; Hydroxyproline; Irradiation; Polymer.

MeSH terms

  • Achilles Tendon / chemistry
  • Amino Acids / chemistry*
  • Animals
  • Bone and Bones / chemistry
  • Bone and Bones / radiation effects
  • Cattle
  • Collagen / chemistry*
  • Fishes
  • Skin / chemistry

Substances

  • Amino Acids
  • Collagen