Isolation of Two Early B Lymphocyte Progenitors From Mouse Marrow: A Committed pre-pre-B Cell and a Clonogenic Thy-1-lo Hematopoietic Stem Cell

Cell. 1986 Feb 28;44(4):653-62. doi: 10.1016/0092-8674(86)90274-6.

Abstract

Two novel early B lymphocyte precursor populations have been identified by their capacity to differentiate in Whitlock-Witte bone marrow cultures. Cells expressing neither the B lineage antigen B220 nor Thy-1 contain committed B cell precursors which differentiate in short-term culture into pre-B and B cells. The other population expresses low levels of Thy-1, and lacks B220 as well as the T cell markers L3T4 and Lyt-2. The Thy-1+ cells which initiate long-term B cell cultures contain clonogenic B cell precursors at a frequency of 1 in 11, a 100-fold enrichment over unseparated bone marrow. Thy-1+ cells are also highly enriched for myeloid-erythroid precursors (CFU-S). Thy-1+ cells allow long-term survival of lethally irradiated mice and fully reconstitute the hematopoietic system, including T and B lymphocyte compartments. These results indicate that this population (approximately 0.1% of bone marrow) may contain the pluripotent hematopoietic stem cell.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigens, Surface / analysis*
  • B-Lymphocytes / cytology*
  • Bone Marrow Cells*
  • Cell Differentiation
  • Colony-Forming Units Assay
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Mice
  • Spleen / cytology
  • Thy-1 Antigens

Substances

  • Antigens, Surface
  • Thy-1 Antigens