The Complete Genome Sequence of Bacillus Velezensis Strain GH1-13 Reveals Agriculturally Beneficial Properties and a Unique Plasmid

J Biotechnol. 2017 Oct 10;259:221-227. doi: 10.1016/j.jbiotec.2017.06.1206. Epub 2017 Jul 6.

Abstract

The bacterial strain Bacillus velezensis GH1-13, isolated from rice paddy soil in Korea, has been shown to promote plant growth and have strong antagonistic activities against pathogens. Here, we report the complete genome sequence of GH1-13, revealing that it possesses a single 4,071,980-bp circular chromosome with 46.2% GC-content. The chromosome encodes 3,930 genes, and we have also identified a unique plasmid in the strain that encodes a further 104 genes (71,628bp and 31.7% GC-content). The genome was found to contain various enzyme-encoding operons, including indole-3-acetic acid (IAA) biosynthesis proteins, 2,3-butanediol dehydrogenase, various non-ribosomal peptide synthetases, and several polyketide synthases. These properties are responsible for the promotion of plant growth and the biosynthesis of secondary metabolites. They therefore have multiple beneficial effects that could be applied to agriculture. Through curing, we found that the unique plasmid of GH1-13 has important roles in the production of phytohormones, such as IAA, and in shaping phenotypic and physiological characteristics. The plasmid therefore likely influences the biological activities of GH1-13. The complete genome sequence of B. velezensis GH1-13 contributes to our understanding of this beneficial strain and will encourage research into its development for agricultural or biotechnological applications, enhancing productivity and crop quality.

Keywords: Antifungal activity; Bacillus velezensis GH1-13; Genome sequence; Plant growth promotion; Plasmid curing; Secondary metabolite.

MeSH terms

  • Agriculture*
  • Bacillus* / genetics
  • Bacillus* / metabolism
  • Genome, Bacterial / genetics*
  • Indoleacetic Acids / metabolism
  • Oryza
  • Plant Growth Regulators* / genetics
  • Plant Growth Regulators* / metabolism
  • Plasmids / genetics
  • Plasmids / metabolism
  • Republic of Korea
  • Soil Microbiology

Substances

  • Indoleacetic Acids
  • Plant Growth Regulators
  • indoleacetic acid