Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)-mRNA Targets by PAR-CLiP

Manana Melikishvili; Elena Matveeva; Yvonne Fondufe-Mittendorf

doi:10.1007/978-1-4939-6993-7_15

Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)-mRNA Targets by PAR-CLiP

Methods Mol Biol. 2017;1608:211-228. doi: 10.1007/978-1-4939-6993-7_15.

Authors

Manana Melikishvili¹, Elena Matveeva¹, Yvonne Fondufe-Mittendorf²

Affiliations

¹ Department of Molecular and Cellular Biochemistry, University of Kentucky, BBSRB 273, 741 S. Limestone, Lexington, KY, 40536, USA.
² Department of Molecular and Cellular Biochemistry, University of Kentucky, BBSRB 273, 741 S. Limestone, Lexington, KY, 40536, USA. y.fondufe-mittendorf@uky.edu.

PMID: 28695513
DOI: 10.1007/978-1-4939-6993-7_15

Abstract

There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA-protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

Keywords: Chromatin; PAR-CliP; PARP1; RNA-binding proteins; Splicing; mRNA.

MeSH terms

Adenosine Diphosphate Ribose / metabolism
Animals
Chromatin / metabolism
Gene Expression Regulation / genetics
Gene Expression Regulation / physiology
Humans
Poly (ADP-Ribose) Polymerase-1 / genetics
Poly (ADP-Ribose) Polymerase-1 / metabolism*
Protein Processing, Post-Translational / genetics
Protein Processing, Post-Translational / physiology
RNA Splicing / genetics
RNA Splicing / physiology
RNA, Messenger / metabolism*
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism

Substances

Chromatin
RNA, Messenger
RNA-Binding Proteins
Adenosine Diphosphate Ribose
Poly (ADP-Ribose) Polymerase-1