Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)-mRNA Targets by PAR-CLiP

Methods Mol Biol. 2017;1608:211-228. doi: 10.1007/978-1-4939-6993-7_15.

Abstract

There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA-protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

Keywords: Chromatin; PAR-CliP; PARP1; RNA-binding proteins; Splicing; mRNA.

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism
  • Animals
  • Chromatin / metabolism
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / physiology
  • Humans
  • Poly (ADP-Ribose) Polymerase-1 / genetics
  • Poly (ADP-Ribose) Polymerase-1 / metabolism*
  • Protein Processing, Post-Translational / genetics
  • Protein Processing, Post-Translational / physiology
  • RNA Splicing / genetics
  • RNA Splicing / physiology
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism

Substances

  • Chromatin
  • RNA, Messenger
  • RNA-Binding Proteins
  • Adenosine Diphosphate Ribose
  • Poly (ADP-Ribose) Polymerase-1