Whey Protein Supplementation Enhances Whole Body Protein Metabolism and Performance Recovery after Resistance Exercise: A Double-Blind Crossover Study

Nutrients. 2017 Jul 11;9(7):735. doi: 10.3390/nu9070735.


No study has concurrently measured changes in free-living whole body protein metabolism and exercise performance during recovery from an acute bout of resistance exercise. We aimed to determine if whey protein ingestion enhances whole body net protein balance and recovery of exercise performance during overnight (10 h) and 24 h recovery after whole body resistance exercise in trained men. In a double-blind crossover design, 12 trained men (76 ± 8 kg, 24 ± 4 years old, 14% ± 5% body fat; means ± standard deviation (SD)) performed resistance exercise in the evening prior to consuming either 25 g of whey protein (PRO; MuscleTech 100% Whey) or an energy-matched placebo (CHO) immediately post-exercise (0 h), and again the following morning (~10 h of recovery). A third randomized trial, completed by the same participants, involving no exercise and no supplement served as a rested control trial (Rest). Participants ingested [15N]glycine to determine whole body protein kinetics and net protein balance over 10 and 24 h of recovery. Performance was assessed pre-exercise and at 0, 10, and 24 h of recovery using a battery of tests. Net protein balance tended to improve in PRO (P = 0.064; effect size (ES) = 0.61, PRO vs. CHO) during overnight recovery. Over 24 h, net balance was enhanced in PRO (P = 0.036) but not in CHO (P = 0.84; ES = 0.69, PRO vs. CHO), which was mediated primarily by a reduction in protein breakdown (PRO < CHO; P < 0.01. Exercise decreased repetitions to failure (REP), maximal strength (MVC), peak and mean power, and countermovement jump performance (CMJ) at 0 h (all P < 0.05 vs. Pre). At 10 h, there were small-to-moderate effects for enhanced recovery of the MVC (ES = 0.56), mean power (ES = 0.49), and CMJ variables (ES: 0.27-0.49) in PRO. At 24 h, protein supplementation improved MVC (ES = 0.76), REP (ES = 0.44), and peak power (ES = 0.55). In conclusion, whey protein supplementation enhances whole body anabolism, and may improve acute recovery of exercise performance after a strenuous bout of resistance exercise.

Keywords: dietary protein; ergogenic aid; net protein balance; power; strength.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Athletic Performance / physiology
  • Body Mass Index
  • Cross-Over Studies
  • Diet
  • Dietary Carbohydrates / administration & dosage
  • Dietary Proteins / administration & dosage
  • Dietary Proteins / metabolism*
  • Dietary Supplements*
  • Double-Blind Method
  • Humans
  • Male
  • Models, Theoretical
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / metabolism
  • Nitrogen / urine
  • Resistance Training*
  • Rest
  • Sample Size
  • Whey Proteins / administration & dosage*
  • Young Adult


  • Dietary Carbohydrates
  • Dietary Proteins
  • Whey Proteins
  • Nitrogen