Microscopy and Image Analysis

Curr Protoc Hum Genet. 2017 Jul 11:94:4.4.1-4.4.89. doi: 10.1002/cphg.42.

Abstract

This unit provides an overview of light microscopy, including objectives, light sources, filters, film, and color photography for fluorescence microscopy and fluorescence in situ hybridization (FISH). We believe there are excellent opportunities for cytogeneticists, pathologists, and other biomedical readers, to take advantage of specimen optical clearing techniques and expansion microscopy-we briefly point to these new opportunities. © 2017 by John Wiley & Sons, Inc.

Keywords: digital imaging; fluorescence in situ hybridization; functional genomics; light microscopy.

Publication types

  • Review

MeSH terms

  • Animals
  • Color
  • Cytogenetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Microscopy / instrumentation*
  • Microscopy / methods*
  • Microscopy, Fluorescence
  • Pathology
  • Photography