Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo

Sci Rep. 2017 Jul 11;7(1):5119. doi: 10.1038/s41598-017-05230-2.


Post-translational redox modification of methionine residues often triggers a change in protein function. Emerging evidence points to this reversible protein modification being an important regulatory mechanism under various physiological conditions. Reduction of oxidized methionine residues is catalyzed by methionine sulfoxide reductases (Msrs). Here, we show that one of these enzymes, a selenium-containing MsrB1, is highly expressed in immune-activated macrophages and contributes to shaping cellular and organismal immune responses. In particular, lipopolysaccharide (LPS) induces expression of MsrB1, but not other Msrs. Genetic ablation of MsrB1 did not preclude LPS-induced intracellular signaling in macrophages, but resulted in attenuated induction of anti-inflammatory cytokines, such as interleukin (IL)-10 and the IL-1 receptor antagonist. This anomaly was associated with excessive pro-inflammatory cytokine production as well as an increase in acute tissue inflammation in mice. Together, our findings suggest that MsrB1 controls immune responses by promoting anti-inflammatory cytokine expression in macrophages. MsrB1-dependent reduction of oxidized methionine in proteins may be a heretofore unrecognized regulatory event underlying immunity and inflammatory disease, and a novel target for clinical applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Female
  • Gene Expression Regulation / drug effects
  • Interleukin 1 Receptor Antagonist Protein / genetics
  • Interleukin-10 / genetics
  • Lipopolysaccharides / adverse effects*
  • Macrophages / cytology
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Methionine Sulfoxide Reductases / genetics
  • Methionine Sulfoxide Reductases / metabolism*
  • Mice
  • Phorbol Esters / adverse effects*
  • Signal Transduction
  • Up-Regulation


  • IL10 protein, mouse
  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Lipopolysaccharides
  • Phorbol Esters
  • Interleukin-10
  • 12-O-undecadienoylphorbol-13-acetate
  • Methionine Sulfoxide Reductases
  • MsrB1 protein, mouse