Whole genome sequencing and annotation of halophilic Salinicoccus sp. BAB 3246 isolated from the coastal region of Gujarat

Vishal Mevada; Shradhdha Patel; Jignesh Pandya; Himani Joshi; Rajesh Patel

doi:10.1016/j.gdata.2017.06.006

Whole genome sequencing and annotation of halophilic Salinicoccus sp. BAB 3246 isolated from the coastal region of Gujarat

Genom Data. 2017 Jun 21:13:30-34. doi: 10.1016/j.gdata.2017.06.006. eCollection 2017 Sep.

Authors

Vishal Mevada¹, Shradhdha Patel², Jignesh Pandya², Himani Joshi², Rajesh Patel²

Affiliations

¹ Shri Sarvajanik Science College, Sarvajanik Campus, Mehsana, India.
² Department of Life Sciences, Hem. North Gujarat University, Patan, India.

Abstract

Salinicoccus sp. BAB 3246 is a halophilic bacterium isolated from a marine water sample collected from the coastal region of Gujarat, India, from a surface water stream. Based on 16sRNA sequencing, the organism was identified as Salinicoccus sp. BAB 3246 (Genebank ID: KF889285). The present work was performed to determine the whole genome sequence of the organism using Ion Torrent PGM platform followed by assembly using the CLC genomics workbench and genome annotation using RAST, BASys and MaGe. The complete genome sequence was 713,204 bp identified by with second largest size for Salinicoccus sp. reported in the NCBI genome database. A total of 652 degradative pathways were identified by KEGG map analysis. Comparative genomic analysis revealed Salinicoccus sp. BAB 3246 as most highly related to Salinicoccus halodurans H3B36. Data mining identified stress response genes and operator pathway for degradation of various environmental pollutants. Annotation data and analysis indicate potential use in pollution control in industrial influent and saline environment.