Novel electrochemiluminescence of silver nanoclusters fabricated on triplex DNA scaffolds for label-free detection of biothiols

Lingyan Feng; Li Wu; Feifei Xing; Lianzhe Hu; Jinsong Ren; Xiaogang Qu

doi:10.1016/j.bios.2017.07.016

Novel electrochemiluminescence of silver nanoclusters fabricated on triplex DNA scaffolds for label-free detection of biothiols

Biosens Bioelectron. 2017 Dec 15:98:378-385. doi: 10.1016/j.bios.2017.07.016. Epub 2017 Jul 6.

Authors

Lingyan Feng¹, Li Wu², Feifei Xing³, Lianzhe Hu⁴, Jinsong Ren², Xiaogang Qu⁵

Affiliations

¹ Materials Genome Institute, Shanghai University, 200444 Shanghai, China. Electronic address: lingyanfeng@t.shu.edu.cn.
² Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China.
³ Department of Chemistry, College of Science, Shanghai University, 200444 Shanghai, China.
⁴ Chongqing Key Laboratory of Green Synthesis and Applications, and Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
⁵ Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China. Electronic address: xqu@ciac.ac.cn.

PMID: 28709087
DOI: 10.1016/j.bios.2017.07.016

Abstract

Electrochemiluminescence (ECL) of metal nanoclusters and their application have been widely reported due to the good biocompatibility, fascinating electrocatalytic activity and so on. Using DNA as synthesis template opens new opportunities to modulate the physical properties of AgNCs. Triplex DNA has been reported for the site-specific, homogeneous and highly stable silver nanoclusters (AgNCs) fabrication from our recent research. Here we further explore their extraordinary ECL properties and applications in biosensor utilization. By reasonable design of DNA sequence, AgNCs were obtained in the predefined position of CG.C⁺ sites of triplex DNA, and the ECL emission at a low potential was observed with this novel DNA template. Finally, a simple and label-free method was developed for biothiols detection based on the enhanced catalytic reaction and a robust interaction between the triplex-AgNCs and cysteine, by influencing the microenvironment provided by DNA template.

Keywords: Biothiols detection; Electrochemical biosensor; Electrochemiluminescence; Silver nanoclusters; Triplex DNA.

MeSH terms

Biosensing Techniques*
DNA / chemistry*
DNA / genetics
Electrochemical Techniques
Fluorescent Dyes
Metal Nanoparticles / chemistry*
Silver / chemistry
Sulfhydryl Compounds / chemistry
Sulfhydryl Compounds / isolation & purification*

Substances

Fluorescent Dyes
Sulfhydryl Compounds
triplex DNA
Silver
DNA