Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures

Denis Saulnier; Sophie De Decker; Delphine Tourbiez; Marie Agnès Travers

doi:10.1016/j.mimet.2017.07.002

Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures

J Microbiol Methods. 2017 Sep:140:67-69. doi: 10.1016/j.mimet.2017.07.002. Epub 2017 Jul 12.

Authors

Denis Saulnier¹, Sophie De Decker², Delphine Tourbiez², Marie Agnès Travers²

Affiliations

¹ Ifremer, UMR 241 EIO, UPF-ILM-IRD, B.P. 49, 98719 Taravao, Tahiti, French Polynesia; Laboratoire d'Excellence "CORAIL", 98729, Moorea, French Polynesia. Electronic address: denis.saulnier@ifremer.fr.
² Laboratoire de Génétique et Pathologie des Mollusques Marins, SG2M-RBE Ifremer, av du Mus de Loup, 17390 La Tremblade, French Polynesia.

PMID: 28709898
DOI: 10.1016/j.mimet.2017.07.002

Abstract

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

Keywords: Gene sequencing; Molluscs; Multiplex real-time PCR; Splendidus clade; Vibrio aestuarianus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Crassostrea / microbiology*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Vibrio / classification*
Vibrio / genetics
Vibrio / isolation & purification*