CRISPR/Cas9-Based Genome Editing in Plants

Prog Mol Biol Transl Sci. 2017;149:133-150. doi: 10.1016/bs.pmbts.2017.03.008. Epub 2017 May 12.

Abstract

Recently, genome editing technologies have shown great potential in plants. The newly developed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a new generation of genome editing tool rapidly replacing the earlier zinc finger nucleases and transcription activator-like effector nucleases systems. Indeed, due to its advantages of simplicity and high efficiency, the CRISPR/Cas9-based genome editing system is becoming a powerful tool in plant science research. Here, we introduce the technical features of the plant CRISPR/Cas9-based genome editing system and its applications in plant functional genomics studies and genetic improvement.

Keywords: CRISPR/Cas9; DSDecode; Genome editing; Plant.

Publication types

  • Review

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Crops, Agricultural / genetics
  • Gene Editing*
  • Genome, Plant*
  • Mutation / genetics
  • Plants / genetics*