New approach for the diagnosis of histamine intolerance based on the determination of histamine and methylhistamine in urine

J Pharm Biomed Anal. 2017 Oct 25;145:379-385. doi: 10.1016/j.jpba.2017.06.029. Epub 2017 Jul 5.

Abstract

Histamine intolerance is a disorder in the homeostasis of histamine due to a reduced intestinal degradation of this amine, mainly caused by diamine oxidase (DAO) enzyme deficiency, which provokes its accumulation in plasma and the appearance of adverse health affects. A new approach for the diagnosis of this intolerance could be the determination of histamine and its metabolites in urine. The aim of this work was to develop and validate a rapid method to determine histamine and methylhistamine in human urine by Ultra High Performance Liquid Chromatography and Fluorimetric detection (UHPLC-FL). The proposed method is a consistent procedure to determine histamine and methylhistamine in less than 11min with adequate linearity and sensitivity. Relative standard deviation was always lower than 5.5%, ensuring method precision; and mean recovery was greater than 99% for both analytes. The structure of histamine and methylhistamine conjugated with OPA were confirmed by UHPLC-ITD-FTMS which enabled to unequivocally identify both analytes in standards and also in urine samples. The analysis of histamine and methylhistamine in urine samples could be a potential new approach for the routine diagnosis of histamine intolerance, more patient-friendly and with clear advantages in terms of equipment and personnel demand for sample collection in comparison with current plasmatic DAO activity determination.

Keywords: Diamine oxidase; Histamine; Histamine intolerance; Methylhistamine; Solid phase extraction; Ultra high pressure liquid chromatography.

MeSH terms

  • Body Fluids
  • Chromatography, High Pressure Liquid
  • Fluorometry
  • Histamine / urine*
  • Humans
  • Methylhistamines

Substances

  • Methylhistamines
  • Histamine