Simple generation of hairless mice for in vivo imaging

Exp Anim. 2017 Oct 30;66(4):437-445. doi: 10.1538/expanim.17-0049. Epub 2017 Jul 18.


The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hrhr mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mice carrying both the reporter gene and mutant Hrhr gene by mating. In addition, there is a risk that genetic background of the gene-modified mice would be altered by mating. To resolve these issues, we established a simple method to generate hairless mice maintaining the original genetic background by CRISPR technology. First, we constructed the pX330 vector, which targets exon 3 of Hr. This DNA vector (5 ng/µl) was microinjected into the pronuclei of C57BL/6J mice. Induced Hr gene mutations were found in many founders (76.1%) and these mutations were heritable. Next, we performed in vivo imaging using these gene-modified hairless mice. As expected, luminescent objects in their body were detected by in vivo imaging. This study clearly showed that hairless mice could be simply generated by the CRISPR/Cas9 system, and this method may be useful for in vivo imaging studies with various gene-modified mice.

Keywords: CRISPR/Cas9; hairless; in vivo imaging; mouse.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • CRISPR-Cas Systems*
  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • DNA / genetics
  • Diagnostic Imaging / methods*
  • Genes, Reporter / genetics
  • Genetic Vectors
  • Mice, Hairless / genetics*
  • Mice, Inbred C57BL
  • Microinjections
  • Mitochondrial Replacement Therapy / methods*
  • Mutation*
  • Phenotype
  • Transcription Factors / genetics*


  • Transcription Factors
  • hr protein, mouse
  • DNA